Observing neural signals in real time offers an important glimpse into how brains perceive the outside world. In the new study, researchers developed a way to follow these signals in the brain of a zebrafish larva, using a sensitive fluorescent marker.
"It's a breakthrough," molecular and cell biologist Florian Engert of Harvard University, who was not involved in the study, told LiveScience. "No one else can look at neuronal activity with fluorescence microscopy in a freely swimming zebrafish larva" with such good resolution.
Zebrafish are widely used to study genetics and development in vertebrates. Their larvae are ideal for neuroimaging because they have translucent heads, and scientists can literally peer into their brains.
To see what was actually going on in those fish noggins, researchers developed a genetically engineered protein, called GCaMP7a, that lights up under a fluorescent microscope when neurons, or brain cells, fire. Transgenic zebrafish were bred to express this protein in a brain region called the optic tectum, which controls the movement of the eye when the animal sees something move in its environment.